Table 2.
Translation Initiation Sequences Surrounding VP1, VP2, and VP3 Start Codons in AAV Serotypes
| AAV Serotype | VP1 ORF |
VP2 ORF |
VP3 ORF |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Up | Kozak TIS, % | Down | Up | Kozak TIS, % | Down | Up | Kozak TIS, % | Down | |
| 1 | CCAGGT | ATG (130) | GC | GCTAAG | ACG | GC | ACTACA | ATG (115) | GC |
| 2 | TCAGGT | ATG (133) | GC | GTTAAG | ACG | GC | AATACG | ATG (114) | GC |
| 3 | CCAGGT | ATG (130) | GC | GCTAAA | ACG | GC | AATACA | ATG (108) | GC |
| 4 | CCAGAT | ATG (99) | AC | GGTGAG | ACG | GC | AGTGAG | ATG (80) | CG |
| 5 | GTAGTC | ATG (91) | TC | GCTAAG | ACG | GC | GATACA | ATG (114) | TC |
| 6 | TTTAAA | ATG (126) | GC | GCTAAG | ACG | GC | ACTACA | ATG (115) | GC |
| 7 | CCAGGT | ATG (130) | GC | GCTAAG | ACG | GC | GGTACA | GTGa | GC |
| 8 | CCAGGT | ATG (130) | GC | GCTAAG | ACG | GC | AATACA | ATG (108) | GC |
| 9 | TCAGGT | ATG (133) | GC | GCTAAG | ACG | GC | CTTACA | ATG (129) | GC |
| 10 | CCAGGT | ATG (130) | GC | GCTAAG | ACG | GC | GGTACA | ATG (113) | GC |
| 11 | CCAGGT | ATG (130) | GC | GCTAAA | ACG | GC | ATTGAA | ATG (81) | CG |
ORF, opened reading frame.
a
Designates a putative alternative initiation codon VP3 in AAV7 deduced by sequences alignment of AAV serotypes capsid genes. The relative strength of Kozak TIS is expressed as % next to the initiation codon.18