Table 1. Effect of TAPP-CZ on C48/80-induced mast cell degranulation.
| Treatment | MTT assay | No. of RPMCs | ||
|---|---|---|---|---|
| OD at 540 nm | % Viability | Degranulated | Intact | |
| Normal | 0.19±0.01 | 100.00 | 9.60±2.50 | 90.40±2.50 |
| C48/80-treated control | 0.19±0.01 | 100.00 | 48.33±5.35### | 51.67±5.346### |
| DSCG (10 µg/ml)+C48/80 | 0.13±0.00 | 72.65 | 18.33±3.84*** | 81.67±3.83*** |
| DSCG (100 µg/ml) | 0.17±0.01 | 91.89 | 0.00±0.00*** | 100.00±0.00*** |
| TAPP-CZ (1 µg/ml)+C48/80 | 0.14±0.01 | 73.30 | 24.33±5.21** | 75.67±5.20** |
| TAPP-CZ (3 µg/ml)+C48/80 | 0.16±0.01 | 86.92 | 14.33±3.66*** | 85.67±3.65*** |
| TAPP-CZ (10 µg/ml)+C48/80 | 0.14±0.02 | 75.68 | 9.00±2.79*** | 91.00±2.79*** |
| TAPP-CZ (30 µg/ml)+C48/80 | 0.17±0.01 | 91.68 | 9.00±2.77*** | 91.00±2.77*** |
TAPP-CZ, type-A procyanidine polyphenols from Cinnamomum zeylanicum; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; RPMC, rat peritoneal mast cell; DSCG, disodium cromoglycate; C48/80, compound 48/80. Data was analyzed by one-way ANOVA followed by Dunnett's test. **P<0.01, ***P<0.001 as compared to C48/80 control group and ###P<0.001 as compared to normal group.