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. 2018 Jan 5;5:2. doi: 10.1186/s40634-017-0118-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Development of the tissue-engineered construct (TEC). a Macroscopic view of the TEC that was integrated to one spherical body. b Hematoxylin and eosin staining of TEC. c Immunohistochemical analysis of the TEC stained with type I collagen (Col I), type II collagen (Col II), type III collagen (Col III), fibronectin, vitronectin, and negative IgG (control). Red are nuclei and green is target antibody. Adhesion molecules such as fibronectin and vitronectin are diffusely distributed within the TEC. Bar = 100 μm. Quoted and modified from Ando et al., Biomaterials 2007 and Shimomura et al. Cartilage 2015