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. 2018 Jan 11;8:1915. doi: 10.3389/fimmu.2017.01915

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Copyright © 2018 Hussain, Zhao, Shah, Wang, Yue, Liao, Sabir, Yang and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mycobacterium avium subspecies paratuberculosis (MAP) infection decreases miR-27a expression in macrophages and mice. (A,B) Bone marrow-derived macrophage (BMDM) and (D,E) RAW264.7 cells were infected with MAP (k-10 and 0908) for the indicated time period, and miR-27a expression was subsequently evaluated by using qRT-PCR. (C) RAW264.7 and (F) BMDMs were stimulated with 1 µg/ml Pam3Cys-Ser-(Lys)4 [PAM toll-like receptor (TLR)1/2 agonist] for the indicated time period, and miR-27a expression was examined using qRT-PCR. (G–I) The expression levels of miR-27a were measured in the intestine (ilium) (G), spleen (H), and liver (I) of negative control or MAP (0908)-infected C57BL/6 mice by qRT-PCR analysis. All data above represent mean ± SD for three independent experiments. *p < 0.05 and **p < 0.001.