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. 2018 Jan 11;8:1915. doi: 10.3389/fimmu.2017.01915

Figure 6.

Figure 6

miR-27a modulates mitogen-activated protein kinase (MAPK) signaling cascades by targeting TGF-β-activated protein kinase 1 binding protein 2 (TAB 2) and TAB 3 in Mycobacterium avium subspecies paratuberculosis (MAP)-infected macrophages. (A) Bone marrow-derived macrophage (BMDM) cells were transfected with 50 nM miR-27a control, mimic, and inhibitor for 48 h and then infected with MAP (0908) at multiplicity of infection (MOI) of 20:1 (bacteria/cell) for 30 min, 6 h, and 18 h. The expression levels of TAB 2, TAB 3, p-P38, p-JNK, and p-ERK were detected by Western blot. (B–F) Western blot analysis of protein levels of TAB 2 (B), TAB 3 (C), p-P38 (D), p-JNK (E) s, and p-ERK (F) normalized to GAPDH after transfection with miR-27a control, mimic, and inhibitor for the indicated times. Data represent mean ± SD from three independent experiments. *p < 0.05, **p < 0.001, and ***p < 0.001.