Figure 1.

Bactericidal effect of bLF and LF-peptides on Vibrio cholerae O1 and non-O1. Approximately 1 × 10⁷ CFU/ml of V. cholerae O1 and non-O1 strains were incubated with bLF and LFpeptides solutions at final concentrations of 40 μM bLF or 20 μM of LFcin17-30, LFampin265-284, respectively; and 5 μM of LFchimera at 37°C with constant agitation for 1, 2, 4, or 6 h. Bacteria grown in LB broth were used as a control for optimal growth and 100 μM of Gentamicin was used as a control for growth inhibition. Bacterial growth was followed by measuring the OD660 nm of cultures. Percentage of viable cells was determined in relation to cultures Gentamicin without peptides or antibiotics (A). All Experiments were repeated at least twice in triplicates. V. cholerae O1 (B) and non-O1 (C) strains were incubated with bLF and LFpeptides solutions at final concentrations of 40 μM bLF and 20 μM LFcin17-30, LFampin265-284, and 5 μM LFchimera; respectively. Viability was monitored by enumerating colony forming units CFU/ml (viable cells) obtained from serial 10-fold dilutions plated onto MH agar (B,C). Percentage of viable cells was calculated relative to viable bacteria untreated grown in MH agar. Experiments were performed in triplicate; mean and standard deviation are indicated. Statistical significance was determined using a Student's t-test for P-values < 0.05, and ANOVA (with Bonferroni correction).