Figure 5.

Interaction of Lactoferrin derived peptides with Vibrio cholerae O1 and non-O1 strains. Vibrio cholerae O1 cells (10⁷ CFU/ml) were incubated with 2 μM FITC-labeled peptides for 30 min. Bacteria were centrifuged (5 min, 10,000 × g), resuspended and incubated with 2 μM of FITC-bLF or FITC-labeled peptides for 30 min (A), or fixed (4% paraformaldehyde, pH 7.4 during 30 min at 37°C) (B), washed and then incubated with 2 μM bLF and LFpeptides as before was described. In both cases samples were washed twice with PBS mounted on slides and processed. All samples were analyzed under confocal microscopy by using a confocal laser-scanning microscope (Leica, Heidelberg, Germany). Bar 20 nm.