Table 1.

Summary of the results of Medihoney and antibiotic treatments on S. aureus.

			Checkerboard combination assay with Medihoney
			Microdilution assayc		MacSynergy II analysisd		Effect on cell viabilitye
	MICa (% w/v or μg/ml)	Biofilm eradicationb (% w/v or μg/ml)	Additive interaction	Honey-antibiotic concentrations with additive interaction (% w/v–μg/ml)	Overall MacSynergy conclusion	Honey-antibiotic concentrations with significant synergistic peaks (% w/v–μg/ml)
Medihoney	8	16	N/A	N/A	N/A	N/A	N/A
Rifampicin	0.04	>4	Yes	8–0.01 8–0.02	Strongly synergistic	1–0.01 2–0.04 4–0.01 4–0.04 8–0.01	Significant
Clindamycin	0.08	0.8	Yes	8–0.02 8–0.04	Antagonistic	2–0.04 2–0.32	Significant
Fusidic acid	0.08	2.4	Yes	8–0.02 8–0.04	Synergistic, minor	8–0.02 8–0.04 8–0.16	Not significant
Gentamicin	0.626	>62.5	No	N/A	Antagonistic	N/A	N/A
Oxacillin	0.16	0.48	No	N/A	Antagonistic	N/A	N/A

^aMIC: minimum inhibitory concentration, expressed as the mean % weight per volume for honey or μg/ml for antibiotics from three separate experiments.

^bData presented in Figure 1. Results expressed as concentration of agent required to achieve ≥85% eradication compared to the untreated control, % weight per volume for honey or μg/ml for antibiotics.

^cData presented in Figure 2, concentrations expressed as % weight per volume for honey and μg/ml for antibiotic.

^dData presented in Figure 3, concentrations expressed as % weight per volume for honey and μg/ml for antibiotic.

^eData presented in Figure 4, left panel. Statistical significance determined using one-way ANOVA, p < 0.05.

N/A, not applicable.