Table 1.

Strains and plasmids used in this work.

Strains/plasmids	Modification	Relevant features∗	Reference
Strain
4/74	None	Wild-type. Virulent reference strain	Wallis et al., 1995
4/74ΔzntA	zntA::Apr	Defective in efflux of lead, cadmium, zinc, and mercury	This work
4/74ΔzitB	zitB::Apr	Defective in zinc efflux across the cytoplasmic membrane	This work
4/74ΔzntA/zitB	zntA::Apr zitB::Chl	Defective in transport of zinc, lead, cadmium, and mercury	This work
4/74ΔfieF	fief::Apr	Defective in a cation-efflux pump FieF induced by iron and zinc	This work
4/74ΔssaV	ssaV::Kn	SPI2-T3SS defective	Unpublished
Plasmid
pKD46	Ap	Plasmid with λ red recombinase expressed from arabinose inducible promoter	Doublet et al., 2008
pUO9090	Apr	Template used in lambda red PCR	Unpublished
pKD3	Chl	Template used in lambda red PCR	Datsenko and Wanner, 2000

Ap, ampicillin; Apr, apramycin; Chl, chrolamphenicol; Kn, kanamycin. ^∗According to the literature.