Figure 6.

Ciliogenesis and Synaptogenesis

(A) γ-Tubulin (green) and ADP ribosylation factor-like GTPase 13b (ARL13B, red) are markers for the cilia basal body and elongated axoneme, respectively.

(B) RHO (green) and ADP ribosylation factor-like GTPase 13b (ARL13B, red) are markers for the rod photoreceptors and the cilia elongated axoneme, respectively.

(C) Bassoon (red) and Synaptophysin (green) are markers for ribbon synapses and synaptic vesicles, respectively. Nuclei were stained with DAPI (blue). Representative figures shown. Arrowheads indicate relevant immunostaining with (A) γ-tubulin, ARL13B, (B) RHO, ARL13B, (C) Bassoon and Synaptophysin. AP shows the apical side of the organoids.

Scale bars, 10 μm.