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. 2018 Jan 15;8:773. doi: 10.1038/s41598-017-19052-9

Table 1.

Primer list.

No Oligo name Gene Sequence Purpose
1 mPerk.SP1 Perk aaggaccctatcctcctgctgcac Genotyping
2 mPerk.SP2 Perk gctaccggtggatgtggaatgtg Genotyping
3 mPerk.AP1 Perk cggagacagtacaagcgcagatga Genotyping
4 mCol2A1.SP1 Col2a1 gggtctcctgcctcctcctgc RT-PCR
5 mCol2A1.AP1 Col2a1 tcctttctgcccctttggccctaattttcg RT-PCR
6 mAcan.SP1 Acan gtcgggagcagcagtcacatctgagcag RT-PCR
7 mAcan.AP1 Acan ccattcgcctctctcatgccagatc RT-PCR
8 mActB.SP1 Actb ccgccctaggcaccagggtg RT-qPCR
9 mActB.AP1 Actb ggctggggtgttgaaggtctcaaa RT-qPCR
10 mAtf4.SP1 Atf4 atgatggcttggccagtg RT-qPCR
11 mAtf4.AP1 Atf4 ccattttctccaacatccaatc RT-qPCR
12 mGadd34.SP1 Gadd34 tcctctaaaagctcggaaggt RT-qPCR
13 mGadd34.AP1 Gadd34 caaagcggcttcgatctc RT-qPCR
14 mChop.SP1 Chop ccaccacacctgaaagcag RT-qPCR
15 mChop.AP1 Chop tcctcataccaggcttcca RT-qPCR
16 mXbp1s.SP1 Xbp1 gctgagtccgaatcaggtgcagg RT-qPCR
17 mXbp1s.AP1 Xbp1 tccttctgggtagacctctgggag RT-qPCR
18 mEdem1.SP1 Edem1 ggtcttcgaagctacgataagg RT-qPCR
19 mEdem1.AP1 Edem1 gggctgtttggaatcagttatta RT-qPCR
20 mErdj4.SP1 Erdj4 cacaaagatgccttttctaccg RT-qPCR
21 mErdj4.AP1 Erdj4 ttaaacttttcagcttaatgacgtg RT-qPCR
22 mGrp94.SP1 Grp94 acacactaggtcgtggaaca RT-qPCR
23 mGrp94.AP1 Grp94 gtctctgtcttgctactccaca RT-qPCR
24 mGrp78.SP1 Grp78 ctgaggcgtatttgggaaag RT-qPCR
25 mGrp78.Ap1 Grp78 tcatgacattcagtccagcaa RT-qPCR
26 mHerp.SP1 Herp acctgagccgagtctaccc RT-qPCR
27 mHerp.AP1 Herp aacagcagcttcccagaataaa RT-qPCR
28 m18s.SP1 18s gtaacccgttgaaccccatt RT-qPCR
29 m18s.AP1 18s ccatccaatcggtagtagcg RT-qPCR
30 rPerk-sgRNA.SP1 Perk catcggatacggcatttggc sgRNA
31 rPerk-sgRNA.AP1 Perk gccaaatgccgtatccgatg sgRNA
32 rPerk-sgRNA.SP2 Perk agatggacgaattgccgcac sgRNA
33 rPerk-sgRNA.AP2 Perk gtgcggcaattcgtccatct sgRNA
34 rPerk-sgRNA.SP3 Perk catacgggctcagtgcatat sgRNA
35 rPerk-sgRNA.AP3 Perk atatgcactgagcccgtatg sgRNA