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. 2018 Jan 15;8:720. doi: 10.1038/s41598-017-18956-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RMSF per residue. RMSF for both J-PKAcα chimera (a) and wild-type PKAcα (b). Appended residues in the J-domain of J-PKAcα are numbered negative with residue 15 the first residue that is common to both J-PKAcα chimera and wild-type PKAcα. (c) Difference in RMSF (chimera – wild-type) beginning at residue 15 where the residues of the chimera and wild-type are equivalent. Positive numbers indicate greater fluctuations in residues of the chimera while negative numbers indicate greater fluctuation in the wild-type PKAcα.