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. 2018 Jan 15;8:737. doi: 10.1038/s41598-017-18658-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Omitting serum during differentiation reduces the number of non-fused cells. Myoblasts were grown to subconfluence and subsequently differentiated for five days in medium with 2% FBS or without FBS. (a) Cells were subsequently stained for skeletal muscle myosin (green, MYH7 or MYH1 + 2) or CD56 (green) and TOPRO (red, nuclei) and images were obtained at 100x magnification. (b–d) mRNA abundance of PPARGC1A, MYH1 and MYH7 related to RPS13 as measured by qPCR. Lines indicate points belonging to the same biopsy. Two-sided, paired t-test. n = 4; **p < 0.01.