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. 2018 Jan 15;8:737. doi: 10.1038/s41598-017-18658-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mitochondria in myotubes are more abundant and qualitatively different from mitochondria in myoblasts. Oxygen consumption of myoblasts and myotubes was measured according to the scheme in Fig. 2. Shown are only cells without TGFβ1 treatment. (a) Respiration normalised to total protein content post measurement. (b) Respiration normalised to total CS content post measurement. (c) CS content normalised to total protein content, both after measurement, and standardised to myoblasts. (d) Relative increase in respiration by succinate addition (e) β-oxidation respiration relative to OXPHOS capacity. (f) Respiration after addition of pyruvate relative to OXPHOS capacity. (g) Respiration after addition of rotenone relative to OXPHOS capacity. (h) Maximal FCCP respiration relative to OXPHOS capacity. (a,b) Repeated measures two-way ANOVA. (c to h) Paired, two-sided t-tests. *p < 0.05, **p < 0.01, ***p < 0.01. n = 4, mean ± SD.