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. 2018 Jan 15;8:757. doi: 10.1038/s41598-018-19158-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Agrobacterium-mediated infiltration assays of RxLR_PVITv1008311 in Vitis vinifera and V. riparia. The relative RxLR PVITv1008311 expression levels were measured during the infection of V. vinifera (a) at different hours post-infection (hpi), in sporangia (b) and one week after infiltration with the RxLR effector PVITv1008311 or the empty vector in V. vinifera (c) and V. riparia (d). The error bars of the relative abundance of the transcript normalized to the P. viticola elongation factor eIF1b in each panel represent the standard deviation of three independent plants or experiments. The necrosis visible on V. riparia leaf in panel (d) is due to dead cells stained in dark blue after trypan blue staining (e). The scale bar represents 5 mm in panels c, d, e and 20 μm in the microscopic pictures in (e).