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. 2018 Jan 15;8:752. doi: 10.1038/s41598-017-19023-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental design for mesencephalic reticular formation stimulation (Experiment 2). In Phase I, we determined the lowest stimulation amplitude that suppressed movement in the home cage, using three different pulses (BP, IPG60, IPG120). The rats were then transferred to the open field, where they received stimulation using this particular pulse shape and amplitude, alternated by OFF conditions. On each test day, only one pulse was evaluated. In total, there were 6 test days for each rat during which each pulse-shape was tested twice. In Phase II, the lowest stimulation amplitude that visibly suppressed mobility was chosen for the IPG120 pulse only. Rats received both BP and IPG120 stimulation in the open field test, using this fixed amplitude. The open field test was repeated the next day, using the same amplitude. Effects on motor behavior were assessed through automated measurements of “Total distance travelled” and manual scores of “%Movement”. Abbreviations: BP = biphasic pulse; IPG60 = interphase gap of 60 µs; IPG120 = interphase gap of 120 µs.