Table 2.
Comparison of different constructs.
| Engineered strain | Plasmid backbone | Expression cassette elements | Selection marker | Activity (U/ml)a |
|---|---|---|---|---|
| YLY1 | pINA1312 | hp4d/lippre-dp-pro-mlip/xpr2t | ura3d1 | 226 ± 4 |
| YLY2 | pINA1317 | hp4d/xpr2pre-dp-pro-mlip/xpr2t | ura3d1 | 1150 ± 5 |
| YLY3 | pINA1317 | hp4d/xpr2pre-pro-mlip/xpr2t | ura3d1 | 1550 ± 3 |
| YLY4 | pINA1317 | hp4d/xpr2pre-mlip/xpr2t | ura3d1 | 987 ± 3 |
| YLY5 | pINA1297 | hp4d/xpr2pre-pro-mlip/xpr2t | ura3d4 | 2175 ± 2 |
aThe engineered strains were cultivated in modified YPD medium. Lipase activity is the mean ± SD of three independent assays.