Fig. 2.

The TADs are demarcated by pairs of insulator proteins. a The locations of known Drosophila insulator proteins, together with the TADs identified in this work, are shown for a 200 kb segment of chr3R. Shown are the positions of class I insulator proteins (that includes BEAF-32 and CP190), as obtained from Flybase, as well as the peak locations of the individual insulator proteins (BEAF-32, CP190, and Chromator) characterized in the modENCODE project. Also shown in the bottom row are the positions of the insulator protein pairs, BEAF-32/CP190, or BEAF-32/Chromator. b Venn diagram showing the genome-wide co-localization of these insulator proteins and insulator protein pairs at the TAD borders. c Logistic regression models to examine the predictive power of the insulator protein pairs (left panel) or transcriptionally active epigenetic modifications or transcriptional levels (right panel) of TAD borders