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. 2018 Jan 15;8:744. doi: 10.1038/s41598-018-19182-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell growth of lymphoma cell lines under hypoxia. (A) Quantitation of live and dead cells in lymphoma cells after incubation in 1% oxygen (hypoxia) for 24 hours using Calcein- AM (Calcein acetoxymethyl ester) and EthD-1 by flow cytometry (FACS CANTO II), n = 3 ± SD. Representative images of live and dead cells in DLBCL cell lines HLY, SUDHL2 and SUDHL6 as determined by flow cytometry are depicted. (B) Results are expressed as the percentage of live cells in DLBCL cell lines under normoxia and hypoxia, respectively.