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. 2018 Jan 15;8:744. doi: 10.1038/s41598-018-19182-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of hypoxia on translation of GLUT1 and HK2 in DLBCL cell lines. Polysomal analysis of GLUT1 and HK2 in HLY, SUDHL2 and SUDHL6 cells maintained under normoxia or hypoxia. RNA was isolated from each polysomal fraction using Trizol and was subjected to RT-PCR analysis. Distribution of (A) GLUT1 and (B) HK2 in different polysomal fractions. Increased GLUT1 and HK2 levels were associated with heavier polysomal fractions ranging from 7–12. (C) DLBCL cells were exposed to normoxia and hypoxia for the indicated time points. The amount of GLUT1 and HK2 protein was detected by immunoblot analysis. GAPDH was used as a loading control. Protein expression for 3, 5, 24 and 48 h was quantitated using Image J software and is depicted in panels below the respective protein blots.