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. 2018 Jan 15;8:701. doi: 10.1038/s41598-018-19209-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Optimisation of CuAAC peptide modification reaction conditions failed to improve H9-OCT4^2AChryIM/w adhesion to pep31-PAPA surfaces. (A) A table shows the reaction conditions that were trialled to improve cellular adhesion to pep31-PAPA surfaces. (B) Counts of mCherry^pos colony numbers on PAPA wells modified with the conditions presented in A. n = 3, Mean +/− SD Images are also presented from day 4 after seeding when (C) rounded colonies of H9-OCT4^2AChryIM/w cells were loosely attached to pep31-PAPA and (D) typical colonies of H9-OCT4^2AChryIM/w cells were attached to cRGDfK-PAPA. Scale bars represent 100 μm.