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. 2018 Jan 15;8:743. doi: 10.1038/s41598-017-16927-9

Figure 6.

Figure 6

MIF exacerbates whereas ISO-1 ameliorates stroke injury in spontaneously hypertensive rat. Permanent cerebral ischemia was induced in SHR by occluding MCA alone (pMCAo). MIF or ISO-1 was injected 3 hours after pMCAo. Rats were then sacrificed 5 hours later in MIF experiments and 21 hours later in ISO-1 experiments. (A) Representative images, summarized results of infarct volume and neurological outcome by exogenous MIF (20 μg/kg, i.v.) administration. Note that MIF increased the infarct volume and neurological score. (B) Representative images, summarized results of infarct volume and neurological outcome by ISO-1 (3 mg/kg, s.c.) administration. Note that ISO-1 decreased the infarct volume and neurological score. The time-course of Evans blue dye assay was shown in the upper panel of (C). Representative images and quantitative results of Evans blue dye extravasation by exogenous MIF (10 μg/kg, i.v.) and ISO-1(3 mg/kg, i.v.) administration were shown in the lower and right panels, respectively. Note that MIF increased and ISO-1 decreased stroke-induced BBB permeability. Data are presented as mean ± S.E.M. (n = 5–7). *p < 0.05 compared with control group. #p < 0.05 compared with MIF treatment group.