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. 2018 Jan 15;8:725. doi: 10.1038/s41598-017-18981-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fibrosis markers decrease in SPARC^−/− mice fed with WD diet. (A,B) Hepatic expression of collagen alpha I, and α-SMA in response to WD feeding compared with controls. *p < 0.05, ***p < 0.001; ^σσp < 0.01; Kruskal-Wallis test with Dunn’s post test. n = 6–8 mice per group. (C) Immunohistochemistry for hepatic α-SMA. Arrowheads indicate the internal positive control-stained area (portal myofibroblast). Arrows indicate α-SMA positive cells in the perisinusoidal zone. (D) α-SMA positive cells quantification. n = 6 mice per group. Twenty random fields were analyzed for each group (x400 magnification) ***p < 0.001, Mann-Whitney U test. (E) Representative images of Sirius red staining of liver sections from SPARC^+/+ and SPARC^−/− mice after feeding WD diet. Arrows indicate collagen deposition. Magnification: 200 × B. (F) Quantification of Sirius red-positive areas using image analysis. n = 6–8 mice per group. **p < 0.01, Kruskal-Wallis test with Dunn’s post test.