Figure 2.

Human NCPCs Express SA Markers and Possess the Positional Identity of Trunk NC Cells

(A) FACS analysis of differentiation of H9 NCPC-4d and NCPC-6d (both representative of ten separations) with heightened expression of NCPC marker p75NTR and SAP marker SA1.

(B) qPCR HOX gene analysis of CNP, NCPC-2d, NCPC-4d, and NCPC-6d. CNP (cranial positional identity, low-number HOX) was used to normalize the expression except for HOXA10, which was normalized to β2M. NCPC/SAP induction is accompanied by decreased expression of lowest-number and increased expression of higher-number HOX paralogs. ND, not detectable, pooled from N = 4 different inductions each, PCRs in triplicate.

Error bars represent mean ± SEM. ns, not significant, ^∗p > 0.05, ^∗∗p > 0.01, ^∗∗∗∗p > 0.0001.