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. 2017 Dec 7;10(1):134–150. doi: 10.1016/j.stemcr.2017.11.003

Figure 4.

Figure 4

Chromaffin-like Cells Differentiated from NCPCs/SAPs Produce CAs

(A) Representative FACS plot of NCPCs differentiated to chromaffin-like cells as analyzed using αTH and PNMT antibodies. The plot suggests emergence of αTH+/PNMT− and αTH+/PNMT+ sub-populations in BMP4-only conditions, while addition of DP reduces the PNMT sub-population. N = 8 independent experiments; error bars represent mean ± SEM.

(B) Immunofluorescence of H9 NCPC-4d cells differentiated with BMP4 (50 pg/mL) for 9 days with the chromaffin markers, PNMT, CgB, and Faglu, markers of CA synthesis and storage. Scale bars, 200 and 5 μm.

(C) HPLC analysis of CA content in lysates of NCPC-4d cells differentiated for 9 days with BMP4 (500 pg/mL), BMP4 (50 pg/mL), DPBMP4 (500 pg/mL), and DPBMP4 (50 pg/mL). BMP4 and corticosteroid mimetic have a stimulatory effect on CA levels. N = 3 independent experiments. Error bars represent mean ± SEM. ∗p > 0.05, ∗∗p > 0.01.