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. 2017 Dec 7;10(1):134–150. doi: 10.1016/j.stemcr.2017.11.003

Figure 7.

Figure 7

NCPC/SAP-like Cells Migrate and Differentiate in Embryonic Tissues

(A) Scheme of the transplantation of hESC-derived NCPCs in quail E2 embryos and incubated in vivo or cultured in vitro or grown on CAM.

(B) Immunofluorescence with chromaffin markers, αTH and PNMT, of NCPCs derived from ENVY-HES3 hESCs transplanted for 4 days in QE2 embryo. This frontal-oblique section is further ventral to the section shown in Figure S7. The human αTH+ cells associate with similar lineage host cells. Scale bars, 50 μm (upper) and 10 μm (lower).

(C) Immunofluorescence with SA markers, αTH and CgB, and human cell-recognizing antibody, anti-human nuclear antigen, of NCPCs derived from H9 hESCs transplanted into QE2 tissue and cultured in vitro for 4 days. Scale bar, 50 μm.

(D) Immunofluorescence with chromaffin markers, αTH and PNMT, and anti-human mitochondria antibody of NCPCs derived from H9 hESCs transplanted into QE2 tissue and cultured on CAM for 8 days. Section is through the αTH-expressing tissue at the margin of mesonephric kidney tissue (arrow). Scale bar, 50 μm.