Comparison of Human iPSC-Derived Organoids with Primary Intestinal Organoids
(A) Images of organoids from the human primary ileum or transverse colon cultured with human organoid culture medium for 6 days. Scale bars, 200 μm.
(B) Relative mRNA levels of the indicated genes in organoids derived from human primary ileum, colon, and iPSCs harvested at 6 days after passage. The relative mRNA levels were determined by qPCR and normalized to GAPDH. The assays were performed in three independent biological replicates. Data are presented as mean ± SEM.