Figure 6.

ESI-017 hNSCs Implanted in HD Mice Increase Expression of BDNF

(A) ESI-017 hNSCs (Ku80, green) show co-localization with BDNF (red); astrocytes are shown as GFAP positive (blue).

(B) Veh-treated mice show no BDNF or hNSCs but have GFAP (blue).

(C) BDNF levels by ELISA in striatum of Q140 or WT mice 6 months post implant.

(D) hNSC treatment in Q140 mice decreased microglial activation. Data are presented as the mean + 95% confidence interval (n = 5 per group). Bars represent percentage of cells of each diameter and the colored portion represents the confidence interval. Significant striatal microglial activation observed in Q140 Veh compared with WT Veh. Q140 hNSC mice showed significant reduction of microglial activation in striatum compared with Q140 Veh mice.

^∗p < 0.05 and ^∗∗p < 0.01 by one-way ANOVA with Bonferroni post hoc test. Graphs show means ± SEM.