Figure 4.

Formation of ATOH1⁺ IEC-Derived Clonal Ribbon Is Significantly Promoted during Regeneration from Colitis-Induced Epithelial Damage

Formation of ATOH1⁺ IEC-derived clonal ribbon (ATOH1⁺ICR) was compared between the DSS-colitis mice (DSS) and control mice (Control).

(A) Experimental design for the lineage-tracing analysis of Atoh1⁺ IECs in DSS-colitis-induced Atoh1^tdTomato mice.

(B) Labeling of ATOH1⁺ IECs-derived cells by tdTomato (red) at day 6 in the colon of DSS-colitis mice and control mice.

(C) Double RNAscope ISH showing expression of Atoh1 (red) and Lgr5 (green) in the colonic crypts of DSS-colitis mice and control mice. Magnified view of the designated area (white square) is shown in the right end column. Images of the colon are re-used in Figure 2C.

(D) An example of ATOH1⁺ICR forming the wound-associated epithelium in DSS-colitis mouse on day 13.

(E) Representative rectal sections showing ATOH1⁺ICRs (red) in the control mice (Control) and in the DSS-colitis mice (DSS) on day 20.

(F) Quantification of the ATOH1⁺ICR formation frequency in the rectum of DSS-colitis mice (n = 3) and control mice (n = 3). The number of ATOH1⁺ICR was normalized by the total number of crypts. Data are expressed as the mean ± SEM per 1,000 crypts. ^∗p < 0.0001.

See also Figure S4.