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. 2017 Dec 14;10(1):287–299. doi: 10.1016/j.stemcr.2017.11.009

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© 2017 The University of Texas M. D. Anderson Cancer Center

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Loss of Gcn5 Leads to Disorganization of Epiblast Cells during Early Differentiation

(A) Bright-field images of differentiating EBs at days 3, 5, and 9, comparing EB morphology between Gcn5^fx/fx and Gcn5^−/− EBs. Scale bar, 200 μM.

(B and C) Representative immunofluorescence/confocal images of EB architecture in day 5 Gcn5^fx/fx and Gcn5^−/− EBs, with (B) Laminin (red) staining for BM and (C) GATA4 (green) staining for VE, and SOX1 (magenta) and DAPI (blue) staining for nuclei of the epiblast. Scale bars, 100 μM in (B) and 50 μM in (C).

See also Figure S1.