Figure 4.

Abnormal Activities of the FGF Signaling Pathway in Day 5 Gcn5^−/− EBs

(A) Representative immunoblots showing decreased phosphorylated forms of ERK and p38 in the Gcn5 null EBs at day 5.

(B) Representative immunoblots showing the AKT pathway is affected to a lesser degree in the null EBs at day 5.

(C) Zeiss LSM 880 confocal Airyscan images showing disorganization of F-actin (red) and vimentin (green) in day 5 null EBs. Scale bars, 10 μM.

(D) Representative immunoblots showing decreased vimentin in Gcn5^−/− EBs at day 5.