Figure 2.

Microarray Analysis Yields Subpopulation-Specific Surface Markers

(A) Gene analysis was performed on the sorted FLK1⁺PDGFRA⁻, FLK1⁺PDGFRA⁺, and CTNT⁺ populations in triplicate. Unsupervised clustering yielded distinct expression patterns unique to each cell type (red, upregulated, white, neutral, blue, downregulated; n = 3 in each group, pooled from 4 independent experiments).

(B) In a comparison between the FLK1⁺PDGFRA⁺ and FLK1⁺PDGFRA⁻ populations, pathway enrichment analysis of the significantly upregulated and downregulated genes revealed pathways relevant to CM development.

(C) Proteins identified by MS from cluster 1 were further segregated as either uniquely or commonly expressed on FLK1⁺PDGFRA⁺ and FLK1⁺PDGFRA⁻ cells using microarray data.