Figure 3.

Flow Cytometry and qPCR Validation of Proteins

(A) FZD4 protein abundance time course was assessed using flow cytometry (n = 4 independent experiments, mean ± SEM).

(B) FLK1⁺PDGFRA⁺ subpopulation concomitantly stained with FZD4 show FZD4⁺ and FZD4⁻ subpopulations. The number in the box represents the percent positive value.

(C) qPCR interrogation of lateral plate and paraxial mesoderm genes on sorted FLK1⁺PDGFRA⁺FZD4⁺ and FLK1⁺PDGFRA⁺FZD4⁻ populations. FLK1⁺PDGFRA⁺FZD4⁺ cells showed an enrichment of lateral plate mesoderm genes while FLK1⁺PDGFRA⁺FZD4⁻ cells showed an enrichment of paraxial mesoderm genes (mean ± SEM, n = 4 independent experiments, ^∗p < 0.05).