Figure 5.

FZD4 Enriches CPC in hPSC-Derived Cardiomyocytes

(A) FZD4⁺ and FZD4⁻ subpopulations were sorted from PDGFRA⁺ cardiac progenitor cells and differentiated into cardiomyocytes. The number in the box represents the percent positive value.

(B and C) Intracellular staining for CTNT (green), DAPI nuclear stain (blue), and Ki67 (purple) (B) shows that the FZD4⁺ compartment exhibited an increased number of cardiomyocytes as compared with the FZD4⁻ population (scale bar represents 500 μm), quantified (C) using CellProfiler (mean ± SEM, n = 3 independent experiments, ^∗p < 0.05).