Figure 2.

Phyl, but Not Sina, Acts as a Limiting Factor for EE Cell Fate Specification

(A and B) Conditional overexpression of phyl in esg⁺ cells for 7 days resulted in excessive EEs (anti-Pros, red) in the midgut.

(C) The percentages of Pros⁺ cells in the epithelia of esg^ts>GFP and esg^ts>phyl midguts. Mean ± SEM. n = 15 for esg^ts>GFP midguts, and n = 16 for esg^ts>phyl midguts. ^∗∗∗∗p < 0.0001 (Student's t test).

(D) Conditional overexpression of phyl in Dl⁺ cells for 7 days also produced excessive EEs. The percentage of Pros⁺ cells in midguts of indicated genotypes. Mean ± SEM. n = 23 for Dl^ts>GFP midguts, and n = 22 for Dl^ts>phyl midguts. ^∗∗∗∗p < 0.0001 (Student's t test).

(E and E′) A flp-out clone (marked by GFP, green) with phyl overexpression co-stained with anti-Pros (red). Dashed lines depict the clone margin.

(F) The phyl overexpression flp-out clones (green) co-stained with anti-PH3 (red).

(G) Epithelium of esg-GAL4^ts>sina midgut stained with anti-Pros (red).

(H) Quantitative data on the percentages of Pros⁺ cells in the epithelia esg^ts>GFP and esg^ts>sina midguts. n = 15 for wild-type control midguts, n = 23 for u-sina midguts.

Scale bars: (A [also applies to B] and G) 20 μm; (E and F) 50 μm. See also Figure S3.