Figure 5.

Phyl Is Transiently Upregulated in EE Progenitor Cells and Is Positively Regulated by Sc

(A–D′) Midguts of indicated genotypes stained with anti-GFP (green). (A) Flies of phyl3.4-nGFP. (B) Flies of Dl-GAL4^ts;u-sc, phyl3.4-nGFP. Flies were shifted to restrictive temperature for 48 hr before analysis. Note that non-specific staining occurred on muscle fibers. Midguts of Dl-GAL4^ts;u-sc, phyl3.4-nGFP stained with Dl and Pros antibody (white) in both the anterior (C) and the posterior (D) midgut regions.

(E) Flies of esg>GFP stained with anti-Pros (white) and anti-Phyl (red) in R3 region. Note that a high expression level of Phyl protein was observed in low-level GFP and Pros⁺ cells.

(F) Quantification of the percentage of indicated cells in R3 region of the midguts of esg>GFP flies. n = 4–6 midguts.

Scale bars: (A, B, and E) 20 μm; (C and D) 10 μm. See also Figure S4.