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. 2018 Jan 14;24(2):226–236. doi: 10.3748/wjg.v24.i2.226

Figure 3.

Figure 3

Hepatitis C virus-1b core protein increases miR-93-5p expression and inactivates the interferon signaling pathway. A: HCV core protein is enforcedly expressed in Huh7 cells using pcDNA3.1 (+) vector. Western blot shows HCV core protein expression. This experiment was repeated twice; B: Bar shows miR-122 expression in Huh7 cells transfected with pcDNA 3.1 (+) empty vector or HCV core-pcDNA3.1 (+) vector. U6 was used as an internal reference. This experiment was repeated three times. C: Bar shows miR-93-5p expression in Huh7 cells transfected with pcDNA 3.1 (+) empty vector or HCV core-pcDNA3.1 (+) vector. U6 was used as an internal reference. This experiment was repeated three times. D and E: Western blot shows the protein expression of IFNAR1 and STAT1, as well as the phosphorylation level of STAT1 in Huh7 cells which were transfected with pcDNA 3.1 (+) empty vector or HCV core-pcDNA3.1 (+) vector. This experiment was repeated twice. HCV: Hepatitis C virus; IFNAR1: Interferon receptor 1.