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. 2018 Jan 14;24(2):226–236. doi: 10.3748/wjg.v24.i2.226

Figure 5.

Figure 5

The miR-93-5p-IFNAR1 axis regulates the interferon signaling pathway. A: Western blot shows the protein expression of IFNAR1 in Huh7 cells transfected with NC (200 ng/mL), siRNA (200 ng/mL), vector (200 ng/mL), or IFNAR1 (200 ng/mL). This experiment was repeated twice; B and C: Western blot shows the phosphorylation level of STAT1 and the protein expression of STAT1 in Huh7 cells transfected with NC (200 ng/mL), siRNA (200 ng/mL), vector (200 ng/mL), or IFNAR1 (200 ng/mL). This experiment was repeated twice; D and E: Western blot shows the phosphorylation level of STAT1 and the protein expression of STAT1 in Huh7 cells transfected with ago-NC (200 nmol/L), miR-93-5p agomir (200 nmol/L), antago-NC (200 nmol/L), or miR-93-5p antagomir (200 nmol/L). This experiment was repeated twice; F and G: Western blot shows the phosphorylation level of STAT1 and the protein expression of STAT1 and IFNAR1 in Huh7 cells transfected with ago-NC (200 nmol/L), miR-93-5p agomir (200 nmol/L), or miR-93-5p agomir (200 nmol/L) plus IFNAR1 (200 ng/mL). IFNAR1: Interferon receptor 1.