Figure 1.

p38α Regulates Mammary Luminal Cell Homeostasis

(A) Immunohistochemistry analysis of p38α expression in mammary ducts from animals of the indicated genotypes. Boxed areas are magnified on the right. Scale bars, 100 μm.

(B) Representative FACS plots showing luminal (EpCAM^highCD49f^med) and basal (EpCAM^medCD49f^high) cell populations in mammary glands from animals of the indicated genotypes.

(C) Quantification of the absolute number of luminal and basal cell populations separated as in (B) (n = 10 animals). ^∗p ≤ 0.05; ^∗∗p ≤ 0.005.

(D) Relative expression of the p38α mRNA in luminal and basal cell populations separated as in (B) from MMTV-Cre mice was determined by qRT-PCR. The expression level in basal cells was given the value of 1.

(E) Genomic DNA was purified from luminal and basal cell populations separated as in (B) from the indicated mice and analyzed by qPCR with primers specific for exon 2 and exon 12 (as a control) of the p38α gene. The relative amount of exon 2 versus exon 12 in cells from MMTV-Cre mice was given the value of 1 (n = 3 animals). ^∗∗p ≤ 0.005; ns, non-significant.

(F) Relative expression of the p38α mRNA in luminal and basal cell populations separated as in (B) from the indicated mice was determined by qRT-PCR (n = 3 animals). The expression levels in cells from MMTV-Cre were given the value of 1. ^∗∗p ≤ 0.005; ns, non-significant.

See also Figure S1.