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. 2017 Dec 28;10(1):257–271. doi: 10.1016/j.stemcr.2017.11.021

Figure 3.

Figure 3

p38α Regulates the ER+ Cell Lineage

(A) FACS plots for luminal cells isolated from the indicated mice and then separated into the ER+ and ER− cell lineages based on Sca1 and CD49b expression. Quantifications are shown in the histogram (n = 7 animals). ∗p ≤ 0.05.

(B) Relative expression of the p38α mRNA in ER+ and ER− cell populations separated as in (A) from MMTV-Cre mice was determined by qRT-PCR. The expression level in ER− cells was given the value of 1.

(C) Relative expression of the p38α mRNA in ER+ and ER− cell populations separated as in (A) from the indicated mice was determined by qRT-PCR (n = 3 animals). The expression levels in cells from MMTV-Cre were given the value of 1. ∗∗p ≤ 0.005.

(D) ER (red) and Keratin5 (green) stainings in virgin females of the indicated genotypes (n = 3 animals). Arrowheads indicate ER+ cells. Scale bars, 20 μm. Quantifications are shown in the histogram. ∗p ≤ 0.05; ∗∗∗p ≤ 0.0005.