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. 2017 Dec 28;10(1):257–271. doi: 10.1016/j.stemcr.2017.11.021

Figure 5.

Figure 5

p38α Regulates RUNX1 Expression through MSK1

(A and B) T47D cells were treated with siRNA control or against p38α, and 48 hr later cell lysates were analyzed by immunoblotting using the indicated antibodies (A) or by qRT-PCR with primers to detect the expression levels of the indicated mRNAs (B). The expression level of each gene in siRNA control cells was given the value of 1 (n = 3 independent experiments). ∗p ≤ 0.05; ∗∗∗∗p ≤ 0.00005.

(C) Localization of MSK1 at the RUNX1 promoter of T47D cells based on ChIP-seq data analysis.

(D) Identification of regions containing histone 3 Ser10 (H3S10P) in the RUNX1 promoter of T47D cells treated with short hairpin RNA (shRNA) control or against MSK1, as determined by ChIP-seq data analysis.

(E) Analysis by ChIP-qPCR of H3S10P at the RUNX1 promoter of T47D cells treated with shRNA control or against MSK1 (n = 4 independent experiments). ∗p ≤ 0.05; ns, not significant.

(F) RUNX1 mRNA expression levels were analyzed in WT or MSK1 null T47D cells by qRT-PCR. The expression level in WT cells was given the value of 1 (n = 3 independent experiments). ∗p ≤ 0.05.