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. 2017 Dec 7;10:408. doi: 10.3389/fnmol.2017.00408

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Copyright © 2017 Kinboshi, Mukai, Nagao, Matsuba, Tsuji, Tanaka, Tokudome, Shimizu, Ito, Ikeda, Inanobe, Kurachi, Inoue and Ohno.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of U0126, SB202190, and SP600125 on BDNF mRNA induction by Kir4.1 knockdown in astrocytes. Astrocytes were pretreated with 10 μM U0126, 10 μM SB202190, or 10 μM SP600125 for 30 min, and transfected with Kir4.1 siRNA or a negative control. The mRNA expression of Kir4.1 (A) or BDNF (B) was analyzed by RT-PCR in astrocytes at 24 h after transfection of Kir4.1 siRNA. The mRNA levels are expressed as the ratio to GAPDH mRNA. Each point represents the mean ± SEM of 5–6 separate experiments. ^∗P < 0.05, ^∗∗P < 0.01, significantly different from the negative control.