Fig 6. TAPF prevents TNF-induced cleavage of PARP and caspases 8, 9, 3 and 7 but not cleavage induced by Fas.
NHDF were untreated, incubated with TAPF or incubated with the negative fraction (NEG) prior to the addition of CHX + either TNF (8h) or agonistic Fas antibody (18h) as indicated. Treatment with CHX alone or no treatment whatsoever were performed in parallel as controls. Cells were washed, lysed and equal volumes were analyzed by immunoblotting for the indicated proteins. Only one representative β-actin panel is shown. Data were replicated in two to five independent experiments.