Figure 2.

miRNAs target sequences located in the porcine IFN-β 3′UTR. miRNA recognition elements (MRE) in the porcine IFN-β 3′UTR for porcine let-7b, miR-26a, miR-34a and miR-145, were predicted using miRanda, RNA hybrid and PITA. (A) Porcine IFN-β 3′UTR gene was cloned into the downstream of firefly luciferase in pmirGLO dual firefly/Renilla luciferase miRNA target expression vector, and the expression of pmirGLO-IFN-β 3′UTR luciferase was inhibited in 3D4/21 cells transfected with miRNA mimics at a final concentration of 10 or 40 nM, (B) 3D4/21 cells were co-transfected with pmirGLO-MRE-wtor pmirGLO-MRE-mut and miRNA mimics at 60 nM, the fluorescence results are shown as the ratio of mt to wt MREs (C) the data represent means ± standard error of the mean of three independent experiments. IFN-β, interferon-β; 3′UTR, 3′ untranslated region; MRE, miRNA recognition elements; mt, mutant type; wt, wild-type.