Table III.
Primers for reverse transcription and real-time PCR of porcine IFN-β and miRNAs.
| Primera | Primer sequence (5′→3′)b |
|---|---|
| IFN-β-F | TGCAACCACCACAATTCC |
| IFN-β-R | CTGAGAATGCCGAAGATCTG |
| RPL4-F | CAAGAGTAACTACAACCTTC |
| RPL4-R | GAACTCTACGATGAATCTTC |
| miRNA RT primer | GCGAGCACAGAATTAATACGACTCACTATAGGT |
| let-7b-F | CGGTGAGGTAGTAGGTTGTGTGGTT |
| miR-26a-F | CGGTTCAAGTAATCCAGGATAGGCT |
| miR-34a-F | GGTGGCAGTGTCTTAGCTGGTTGT |
| miR-145-F | GTCCAGTTTTCCCAGGAATCCCT |
| Uni-miRNA-R | GCGAGCACAGAATTAATACGACTCAC |
| U6-F | CTCGCTTCGGCAGCACA |
| U6-R | AACGCTTCACGAATTTGCGT |
a
F, forward PCR primer; R, reverse PCR primer except U6-R acted as reverse transcription primer and reverse PCR primer
b
V, G/A/C; N, A/G/C/T.