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. 2017 Oct 30;15(1):576–584. doi: 10.3892/etm.2017.5400

Figure 3.

Figure 3.

RSV modulates the expression of mitochondrial biogenesis-related, lipid metabolism-related and β-cells-related genes via SIRT1 in INS-1E cells. (A) mRNA and (B) protein levels of PGC-1α and FOXO3a in INS-1E cells. (C) The relative densities of target protein bands were normalized against β-actin. **P<0.01. SIRT1, Sirtuin 1; PGC1α, peroxisome proliferator-activated receptor-γ coactivator-1α; FOXO3a, forkhead box O3a; NC, normal INS-1E cells pretreated with vehicle; HF, INS-1E cells incubated with 0.5 mM PA for 24 h; HF+RSV, INS-1E cells incubated with 0.5 mM PA and 10 µM RSV for 24 h; HF+RSV+SIRT siRNA, INS-1E cells transfected with SIRT1 siRNA for 48 h and incubated with PA 0.5 µM and 10 µM RSV for 24 h; HF+RSV+NC siRNA, INS-1E cells transfected with negative control siRNA for 48 h and incubated with PA 0.5 mM and 10 µM RSV for 24 h.