Figure 5.

mRNA expression of (A) MexZ, MexX, MexY, OprM and OprD in PA012 treated with or without BEB (1/4 MIC) and/or IMP (1/8 MIC) and (B) OprD in PA ATCC27853 and PA012 without antibacterial agent treatments was analyzed by reverse-transcription quantitative polymerase chain reaction. RpsL was used as the reference gene. *P<0.05; **P<0.01; ***P<0.001 vs. the control group. MIC, minimum inhibitory concentration; BEB, berberine; IMP, imipenem; PA, Pseudomonas aeruginosa; ATCC, American Type Tissue Collection; Opr, outer membrane protein.