Figure 1.

Evaluation of the novel three-dimensional tumorsphere culture system. (A) Schematic diagram of the culture system. (B) A photograph of the new culture system in which tumorspheres were suspended without agitation. (C) Differences in the morphology of the tumorspheres in the culture medium with or without MC. (D) Suspension characteristics of tumorspheres in the culture medium with or without 0.03% GG. (E) Size distribution of the tumorspheres derived from 5–8F cells with or without MC. MC, MC, methylcellulose; GG, gellan gum.