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Forward and reverse sequences of the quantitative polymerase chain reaction primers.
| Primer | Forward | Reverse |
|---|---|---|
| Sox2 | 5′-GCCGAGTGGAAACTTTTGTCG-3′ | 5′-GGCAGCGTGTACTTATCCTTCT-3′ |
| Oct4 | 5-CTGGGTTGATCCTCGGACCT-3 | 5-CCATCGGAGTTGCTCTCCA-3 |
| Nanog | 5-TTTGTGGGCCTGAAGAAAACT-3 | 5-GAAGGTGAAGGTCGGAGTC-3 |
| GAPDH | 5-AGGGCTGTCCTGAATAAGCAG-3 | 5-GAAGATGGTGATGGGATTTC-3 |
Sox2, (sex determining region Y)-box 2; Oct4, octamer-binding transcription factor 4.
