Fig. 1.

Saturated Tiling Capture Sequencing (STC-Seq) can efficiently capture the exons (> 23 bp) of all IMGT documented HLA-A/B/C/DQB1/DRB1 alleles. a The experimental pipeline of Saturated Tiling Capture Sequencing (STC-Seq). b Sequence identity between the STC baits and exons (> 23 bp) of all IMGT documented HLA-A/B/C/DQB1/DRB1 alleles. The sequence identity was evaluated based on the number of matched bases normalized by the exon length. c The fold-enrichment of the exon 5 of HLA-A*01:01:01 and HLA-B*73:01 after hybridization capture. The fold-enrichment was measured by real-time PCR. d The exons (> 23 bp) of the HLA-A/B/C/DQB1/DRB1 alleles having the minimum sequence identity with the corresponding STC baits